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Image Search Results
Journal: Journal of Virology
Article Title: The C Terminus of Rotavirus VP4 Protein Contains an Actin Binding Domain Which Requires Cooperation with the Coiled-Coil Domain for Actin Remodeling
doi: 10.1128/JVI.01598-18
Figure Lengend Snippet: Summary of the VP4-GFP constructs used in the present study. Each construct was obtained using the backbone plasmid pEGFP-C1, and all truncated-VP4 constructs encoding amino acid stretches 481 to 776, 481 to 773, 481 to 757, 562 to 776, 641 to 776, 641 to 773, 641 to 757, 702 to 776, 713 to 776, 713 to 773, and 718 to 776, as well as the constructs containing deletions (481-776 del 574-670, 481-776 del 574-713, and 481-776 del 574-718), were generated by PCR and inserted within pEGFP-C1 vector via EcoRI and BamHI sites. Sp100 coiled-coil fused to VP4 (562-776) was made by insertion of PCR-amplified Sp100 coiled-coil 29-152 in VP4 (562-776). Note that GFP-truncated VP4 encoding amino acid stretches 713 to 776, 718 to 776, 718 to 773, and 733 to 776 were made by insertion of amplified segments from the SA11 strain in pEGFP-C1 via XhoI and BamHI sites.
Article Snippet: All constructs encoding GFP-truncated VP4 were made using the
Techniques: Construct, Plasmid Preparation, Generated, Amplification
Journal: eLife
Article Title: ARHGAP18-ezrin functions as an autoregulatory module for RhoA in the assembly of distinct actin-based structures
doi: 10.7554/eLife.83526
Figure Lengend Snippet:
Article Snippet: Recombinant DNA reagent , AHPH-GFP , ,
Techniques: Knock-Out, Recombinant, GAP Assay